KMID : 1094720190240050754
|
|
Biotechnology and Bioprocess Engineering 2019 Volume.24 No. 5 p.754 ~ p.760
|
|
Comparison of Cationic Flocculants for the Clarification of CHO-derived Monoclonal Antibodies
|
|
Carvalho Rimenys Junior
|
|
Abstract
|
|
|
This work used two different cationic polyelectrolytes as flocculation agents for the clarification of growth medium from Chinese hamster ovary (CHO) monoclonal antibodies (mAb)-producing cells, namely chitosan and poly diallyldimethylammonium chloride (pDADMAC). The conditions set for the use of pDADMAC consisted in the variation of the polymer¡¯s concentrations, in light of largely available literature. Chitosan was applied to a broader concentration-range under two different pH values, for its use in CHO cells flocculation is unprecedented in the literature. Chitosan presented the best performance on clarification assays under a concentration 9-fold lower compared with pDADMAC (5 pg/total cell (TC) and 45 pg/TC, respectively), albeit both agents provided similar results regarding sedimentation rates (15 min), DNA removal (98%), and mAb recovery (90%). Despite the expected low proteins removal, namely 11.1% in the case of chitosan and no removal for pDADMAC, the flocculation technique proved to be cost-effective. Hence, its application can be envisioned not only when the clarification of a growth medium from CHO cells production is considered, but also for the removal of soluble impurities in a single step.
|
|
KEYWORD
|
|
Chinese hamster ovary cells, clarification, flocculation, monoclonal antibodies, chitosan, pDADMAC
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|